Preparation and characterization of PLA-PEG/Chitosan-FA/DNA for gene transfer to MCF-7 cells

• When PLA/PEG/Chitosan/FA/DNA nanoparticles interact with MCF-7 cells, their loading percentage and biocompatibility improve. Physicochemical properties of for properly targeted transfer to cells. An increase in Chitosan-FA on gene efficiency was seen positively by nanoparticles. The ability Chitosan-Folic acid PLA-PEG polymers delivery into cells evaluated. copolymer is one the most appealing human because its high blood circulation. However, barriers use these therapy has always been low effectiveness In this study, Chitosan folic were utilized improve encapsulation PLA-PEG/Chitosan-FA/DNA different concentrations prepared using double emulsion-solvent evaporation technique. biological (i.e., biocompatibility, DNA release, vitro) as well physical chemical particle size, zeta potential, morphology resultant nanoparticles) investigated. results showed that increasing concentration resulted an encapsulation, efficiency. MTT assay exhibit cytotoxicity good compatibility. On other hand, fluorescence microscopy flow cytometry used test varied chitosan-folic Flow analysis improved